Molybdenum Trioxide Quantum Dot-Encapsulated Nanogels for Virus Detection by Surface-Enhanced Raman Scattering on a 2D Substrate

Ojodomo J. Achadu; Fuyuki Abe; Tian Cheng Li; Indra Memdi Khoris; Dongkyu Lee; Jaebeom Lee; Tetsuro Suzuki; Enoch Y. Park

doi:10.1021/acsami.1c04793

Molybdenum Trioxide Quantum Dot-Encapsulated Nanogels for Virus Detection by Surface-Enhanced Raman Scattering on a 2D Substrate

Ojodomo J. Achadu, Fuyuki Abe, Tian Cheng Li, Indra Memdi Khoris, Dongkyu Lee, Jaebeom Lee, Tetsuro Suzuki, Enoch Y. Park

SHLS Life Sciences

Research output: Contribution to journal › Article › peer-review

5 Citations (Scopus)

Abstract

The use of nanogels (NGs) to modulate surface-enhanced Raman scattering (SERS) activities is introduced as an innovative strategy to address certain critical issues with SERS-based immunoassays. This includes the chemical deformation of SERS nanotags, as well as their nonspecific interactions and effective "hotspots"formation. Herein, the polymeric cocoon and stimuli-responsive properties of NGs were used to encapsulate SERS nanotags containing plasmonic molybdenum trioxide quantum dots (MoO3-QDs). The pH-controlled release of the encapsulated nanotags and their subsequent localization by maleimide-functionalized magnetic nanoparticles facilitated the creation of "hotspots"regions with catalyzed SERS activities. This approach resulted in developing a biosensing platform for the ultrasensitive immunoassays of hepatitis E virus (HEV) or norovirus (NoV). The immunoassays were optimized using the corresponding virus-like particles to attain limits of detection of 6.5 and 8.2 fg/mL for HEV-LPs and NoV-LPs, respectively. The SERS-based technique achieved a signal enhancement factor of up to ∼108 due to the combined electromagnetic and chemical mechanisms of the employed dual-SERS substrate of MoO3-QDs/2D hexagonal boron nitride nanosheets. The highlight and validation of the developed SERS-based immunoassays was the detection of NoV in infected patients' fecal specimen and clinical HEV G7 subtype. Importantly, this system can be used to maintain the stability of SERS nanotags and improve their reliability in immunoassays.

Original language	English
Pages (from-to)	27836-27844
Number of pages	9
Journal	ACS Applied Materials and Interfaces
Volume	13
Issue number	24
Early online date	9 Jun 2021
DOIs	https://doi.org/10.1021/acsami.1c04793
Publication status	Published - 23 Jun 2021

Bibliographical note

Funding Information:
O.J.A. gratefully thanks the Japan Society for the Promotion of Science (JSPS) for a Postdoctoral Fellowship for Research in Japan (Standard) (Grant No. 19F19348). This work was partly supported by the Japan Agency for Medical Research and Development (20hm0102080h0001).

Publisher Copyright:
© 2021 American Chemical Society.

Access to Document

10.1021/acsami.1c04793Licence: Unspecified

Cite this

@article{65d984b5be6343358dab48c6050010a4,

title = "Molybdenum Trioxide Quantum Dot-Encapsulated Nanogels for Virus Detection by Surface-Enhanced Raman Scattering on a 2D Substrate",

abstract = "The use of nanogels (NGs) to modulate surface-enhanced Raman scattering (SERS) activities is introduced as an innovative strategy to address certain critical issues with SERS-based immunoassays. This includes the chemical deformation of SERS nanotags, as well as their nonspecific interactions and effective {"}hotspots{"}formation. Herein, the polymeric cocoon and stimuli-responsive properties of NGs were used to encapsulate SERS nanotags containing plasmonic molybdenum trioxide quantum dots (MoO3-QDs). The pH-controlled release of the encapsulated nanotags and their subsequent localization by maleimide-functionalized magnetic nanoparticles facilitated the creation of {"}hotspots{"}regions with catalyzed SERS activities. This approach resulted in developing a biosensing platform for the ultrasensitive immunoassays of hepatitis E virus (HEV) or norovirus (NoV). The immunoassays were optimized using the corresponding virus-like particles to attain limits of detection of 6.5 and 8.2 fg/mL for HEV-LPs and NoV-LPs, respectively. The SERS-based technique achieved a signal enhancement factor of up to ∼108 due to the combined electromagnetic and chemical mechanisms of the employed dual-SERS substrate of MoO3-QDs/2D hexagonal boron nitride nanosheets. The highlight and validation of the developed SERS-based immunoassays was the detection of NoV in infected patients' fecal specimen and clinical HEV G7 subtype. Importantly, this system can be used to maintain the stability of SERS nanotags and improve their reliability in immunoassays.",

author = "Achadu, {Ojodomo J.} and Fuyuki Abe and Li, {Tian Cheng} and Khoris, {Indra Memdi} and Dongkyu Lee and Jaebeom Lee and Tetsuro Suzuki and Park, {Enoch Y.}",

note = "Funding Information: O.J.A. gratefully thanks the Japan Society for the Promotion of Science (JSPS) for a Postdoctoral Fellowship for Research in Japan (Standard) (Grant No. 19F19348). This work was partly supported by the Japan Agency for Medical Research and Development (20hm0102080h0001). Publisher Copyright: {\textcopyright} 2021 American Chemical Society.",

year = "2021",

month = jun,

day = "23",

doi = "10.1021/acsami.1c04793",

language = "English",

volume = "13",

pages = "27836--27844",

journal = "ACS Applied Materials and Interfaces",

issn = "1944-8244",

publisher = "American Chemical Society",

number = "24",

}

TY - JOUR

T1 - Molybdenum Trioxide Quantum Dot-Encapsulated Nanogels for Virus Detection by Surface-Enhanced Raman Scattering on a 2D Substrate

AU - Achadu, Ojodomo J.

AU - Abe, Fuyuki

AU - Li, Tian Cheng

AU - Khoris, Indra Memdi

AU - Lee, Dongkyu

AU - Lee, Jaebeom

AU - Suzuki, Tetsuro

AU - Park, Enoch Y.

N1 - Funding Information: O.J.A. gratefully thanks the Japan Society for the Promotion of Science (JSPS) for a Postdoctoral Fellowship for Research in Japan (Standard) (Grant No. 19F19348). This work was partly supported by the Japan Agency for Medical Research and Development (20hm0102080h0001). Publisher Copyright: © 2021 American Chemical Society.

PY - 2021/6/23

Y1 - 2021/6/23

N2 - The use of nanogels (NGs) to modulate surface-enhanced Raman scattering (SERS) activities is introduced as an innovative strategy to address certain critical issues with SERS-based immunoassays. This includes the chemical deformation of SERS nanotags, as well as their nonspecific interactions and effective "hotspots"formation. Herein, the polymeric cocoon and stimuli-responsive properties of NGs were used to encapsulate SERS nanotags containing plasmonic molybdenum trioxide quantum dots (MoO3-QDs). The pH-controlled release of the encapsulated nanotags and their subsequent localization by maleimide-functionalized magnetic nanoparticles facilitated the creation of "hotspots"regions with catalyzed SERS activities. This approach resulted in developing a biosensing platform for the ultrasensitive immunoassays of hepatitis E virus (HEV) or norovirus (NoV). The immunoassays were optimized using the corresponding virus-like particles to attain limits of detection of 6.5 and 8.2 fg/mL for HEV-LPs and NoV-LPs, respectively. The SERS-based technique achieved a signal enhancement factor of up to ∼108 due to the combined electromagnetic and chemical mechanisms of the employed dual-SERS substrate of MoO3-QDs/2D hexagonal boron nitride nanosheets. The highlight and validation of the developed SERS-based immunoassays was the detection of NoV in infected patients' fecal specimen and clinical HEV G7 subtype. Importantly, this system can be used to maintain the stability of SERS nanotags and improve their reliability in immunoassays.

AB - The use of nanogels (NGs) to modulate surface-enhanced Raman scattering (SERS) activities is introduced as an innovative strategy to address certain critical issues with SERS-based immunoassays. This includes the chemical deformation of SERS nanotags, as well as their nonspecific interactions and effective "hotspots"formation. Herein, the polymeric cocoon and stimuli-responsive properties of NGs were used to encapsulate SERS nanotags containing plasmonic molybdenum trioxide quantum dots (MoO3-QDs). The pH-controlled release of the encapsulated nanotags and their subsequent localization by maleimide-functionalized magnetic nanoparticles facilitated the creation of "hotspots"regions with catalyzed SERS activities. This approach resulted in developing a biosensing platform for the ultrasensitive immunoassays of hepatitis E virus (HEV) or norovirus (NoV). The immunoassays were optimized using the corresponding virus-like particles to attain limits of detection of 6.5 and 8.2 fg/mL for HEV-LPs and NoV-LPs, respectively. The SERS-based technique achieved a signal enhancement factor of up to ∼108 due to the combined electromagnetic and chemical mechanisms of the employed dual-SERS substrate of MoO3-QDs/2D hexagonal boron nitride nanosheets. The highlight and validation of the developed SERS-based immunoassays was the detection of NoV in infected patients' fecal specimen and clinical HEV G7 subtype. Importantly, this system can be used to maintain the stability of SERS nanotags and improve their reliability in immunoassays.

UR - http://www.scopus.com/inward/record.url?scp=85108644300&partnerID=8YFLogxK

U2 - 10.1021/acsami.1c04793

DO - 10.1021/acsami.1c04793

M3 - Article

C2 - 34105944

AN - SCOPUS:85108644300

SN - 1944-8244

VL - 13

SP - 27836

EP - 27844

JO - ACS Applied Materials and Interfaces

JF - ACS Applied Materials and Interfaces

IS - 24

ER -

Molybdenum Trioxide Quantum Dot-Encapsulated Nanogels for Virus Detection by Surface-Enhanced Raman Scattering on a 2D Substrate

Abstract

Bibliographical note

Access to Document

Other files and links

Fingerprint

Cite this