Nationwide stepwise emergence and evolution of multidrug-resistant campylobacter jejuni sequence type 5136, united kingdom

Bruno S. Lopes; Norval J.C. Strachan; Meenakshi Ramjee; Anne Thomson; Marion Macrae; Sophie Shaw; Ken J. Forbes

doi:10.3201/eid2507.181572

Nationwide stepwise emergence and evolution of multidrug-resistant campylobacter jejuni sequence type 5136, united kingdom

Bruno S. Lopes, Norval J.C. Strachan, Meenakshi Ramjee, Anne Thomson, Marion Macrae, Sophie Shaw, Ken J. Forbes

SHLS Life Sciences

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Abstract

We examined whole-genome–sequenced Campylobacter jejuni/ and C. coli from 2012–2015 isolated from birds and human stool samples in North East Scotland for the presence of antimicrobial resistance genes. We found that sequence type (ST) 5136 (clonal complex 464) was the most prevalent multidrug-resistant strain of C. jejuni exclusively associated with poultry host reservoirs and recovered from human cases of campylobacteriosis. Tetracycline resistance in ST5136 isolates was due to a tet(O/32/O) mosaic gene, ampicillin resistance was conferred by G →T transversion in the –10 promoter region of blaOXA-193, fluoroquinolone resistance was due to C257T change in gyrA, and aminoglycoside resistance was conferred by aac. Wholegenome analysis showed that the strain ST5136 evolved from ST464. The nationwide emergence of ST5136 was probably due to stepwise acquisition of antimicrobial resistance genes selected by high use of β-lactam, tetracycline, fluoroquinolone, and aminoglycoside classes of drugs in the poultry industry.

Original language	English
Pages (from-to)	1320-1329
Number of pages	10
Journal	Emerging Infectious Diseases
Volume	25
Issue number	7
DOIs	https://doi.org/10.3201/eid2507.181572
Publication status	Published - 29 May 2019

Bibliographical note

Funding Information:
We thank Food Standards Scotland (Contracts S14054, FSS00017) and the Scottish Government's Rural and Environment Science and Analytical Services (RG13588-10) for funding this work. Part of this work was presented at the Campylobacter, Helicobacter and Related Organisms (CHRO) 2017 Congress, Nantes, France.

Publisher Copyright:
© 2019, Centers for Disease Control and Prevention (CDC). All rights reserved.

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title = "Nationwide stepwise emergence and evolution of multidrug-resistant campylobacter jejuni sequence type 5136, united kingdom",

abstract = "We examined whole-genome–sequenced Campylobacter jejuni/ and C. coli from 2012–2015 isolated from birds and human stool samples in North East Scotland for the presence of antimicrobial resistance genes. We found that sequence type (ST) 5136 (clonal complex 464) was the most prevalent multidrug-resistant strain of C. jejuni exclusively associated with poultry host reservoirs and recovered from human cases of campylobacteriosis. Tetracycline resistance in ST5136 isolates was due to a tet(O/32/O) mosaic gene, ampicillin resistance was conferred by G →T transversion in the –10 promoter region of blaOXA-193, fluoroquinolone resistance was due to C257T change in gyrA, and aminoglycoside resistance was conferred by aac. Wholegenome analysis showed that the strain ST5136 evolved from ST464. The nationwide emergence of ST5136 was probably due to stepwise acquisition of antimicrobial resistance genes selected by high use of β-lactam, tetracycline, fluoroquinolone, and aminoglycoside classes of drugs in the poultry industry.",

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AU - Macrae, Marion

AU - Shaw, Sophie

AU - Forbes, Ken J.

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N2 - We examined whole-genome–sequenced Campylobacter jejuni/ and C. coli from 2012–2015 isolated from birds and human stool samples in North East Scotland for the presence of antimicrobial resistance genes. We found that sequence type (ST) 5136 (clonal complex 464) was the most prevalent multidrug-resistant strain of C. jejuni exclusively associated with poultry host reservoirs and recovered from human cases of campylobacteriosis. Tetracycline resistance in ST5136 isolates was due to a tet(O/32/O) mosaic gene, ampicillin resistance was conferred by G →T transversion in the –10 promoter region of blaOXA-193, fluoroquinolone resistance was due to C257T change in gyrA, and aminoglycoside resistance was conferred by aac. Wholegenome analysis showed that the strain ST5136 evolved from ST464. The nationwide emergence of ST5136 was probably due to stepwise acquisition of antimicrobial resistance genes selected by high use of β-lactam, tetracycline, fluoroquinolone, and aminoglycoside classes of drugs in the poultry industry.

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Nationwide stepwise emergence and evolution of multidrug-resistant campylobacter jejuni sequence type 5136, united kingdom

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