TY - JOUR
T1 - NO loading
T2 - Efficiency assessment of five commonly used application methods of sodium nitroprusside in Medicago truncatula plants
AU - Filippou, Panagiota
AU - Antoniou, Chrystalla
AU - Yelamanchili, Shirisha
AU - Fotopoulos, Vasileios
PY - 2012/11/1
Y1 - 2012/11/1
N2 - Nitric oxide (NO) is a bioactive, diffusible molecule involved in a multitude of physiological and developmental processes in plants, which has been reported to display both antioxidant and pro-oxidant properties in plants. Several reports exist highlighting the protective action of sodium nitroprusside (SNP), an NO donor, which demonstrate its important role as a signal molecule in plants responsible for the expression regulation of antioxidant and other defense enzymes. However, the mode of application of this compound varies greatly between studies. The present study provides a comprehensive efficiency comparison of the most commonly used application methods using 2.5. mM SNP on mature (40 day) Medicago truncatula plants. Measurement of NO content in both leaves and roots suggests that vacuum infiltration is the most efficient method for NO donation in leaf tissue, whereas hydroponic application resulted in highest NO content in roots. NO content correlated with activity levels of nitrate reductase (NR; EC 1.7.99.4), a key enzyme involved in the generation of NO in plants and which is known to be regulated by NO itself.
AB - Nitric oxide (NO) is a bioactive, diffusible molecule involved in a multitude of physiological and developmental processes in plants, which has been reported to display both antioxidant and pro-oxidant properties in plants. Several reports exist highlighting the protective action of sodium nitroprusside (SNP), an NO donor, which demonstrate its important role as a signal molecule in plants responsible for the expression regulation of antioxidant and other defense enzymes. However, the mode of application of this compound varies greatly between studies. The present study provides a comprehensive efficiency comparison of the most commonly used application methods using 2.5. mM SNP on mature (40 day) Medicago truncatula plants. Measurement of NO content in both leaves and roots suggests that vacuum infiltration is the most efficient method for NO donation in leaf tissue, whereas hydroponic application resulted in highest NO content in roots. NO content correlated with activity levels of nitrate reductase (NR; EC 1.7.99.4), a key enzyme involved in the generation of NO in plants and which is known to be regulated by NO itself.
UR - http://www.scopus.com/inward/record.url?scp=84865311277&partnerID=8YFLogxK
U2 - 10.1016/j.plaphy.2012.07.026
DO - 10.1016/j.plaphy.2012.07.026
M3 - Article
C2 - 22922111
AN - SCOPUS:84865311277
SN - 0981-9428
VL - 60
SP - 115
EP - 118
JO - Plant Physiology and Biochemistry
JF - Plant Physiology and Biochemistry
ER -