Optical biosensing strategies for DNA methylation analysis

Md Nazmul Islam, Sharda Yadav, Md Hakimul Hague, Ahmed Munaz, Farhadul Islam, Md Shahriar Al Hossain, Vinod Gopalan, Alfred K. Lam, Nam-Trung Nguyen, Muhammad J. A. Shiddiky

    Research output: Contribution to journalArticlepeer-review

    Abstract

    DNA methylation is an epigenetic modification of DNA, where a methyl group is added at the fifth carbon of the cytosine base to form 5 methyl cytosine (5mC) without altering the DNA sequences. It plays important roles in regulating many cellular processes by modulating key genes expression. Alteration in DNA methylation patterns becomes particularly important in the aetiology of different diseases including cancers. Abnormal methylation pattern could contribute to the pathogenesis of cancer either by silencing key tumor suppressor genes or by activating oncogenes. Thus, DNA methylation biosensing can help in the better understanding of cancer prognosis and diagnosis and aid the development of therapies. Over the last few decades, a plethora of optical detection techniques have been developed for analyzing DNA methylation using fluorescence, Raman spectroscopy, surface plasmon resonance (SPR), electrochemiluminescence and colorimetric readouts. This paper aims to comprehensively review the optical strategies for DNA methylation detection. We also present an overview of the remaining challenges of optical strategies that still need to be focused along with the lesson learnt while working with these techniques.
    Original languageEnglish
    Pages (from-to)668-678
    JournalBiosensors & Bioelectronics
    Volume92
    Early online date19 Oct 2016
    DOIs
    Publication statusPublished - 15 Jun 2017

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