Abstract
The transcription factor ACEII (Activator of cellulase 2) of Trichoderma reesei is the first identified transactivator for cellulase and xylanase genes. However, relatively little information is available about the regulatory function of ACEII. In this study, strains expressing tagged-ACEII under the control of the cbh1 and the xyn3 promoters were developed to investigate the effect of overexpression of ACEII. The resultant strains expressed markedly higher amount of ace2 transcript in the cellulase-inducing conditions, and the maximum level of the transcript induction was estimated to be 570-fold for the cbh1 construct and 20-fold for the xyn3 construct. Analysis of production and expression profiles of cellulase revealed the possible involvement of ACEII in the regulation of cellulase and xylanase gene expressions in T. reesei. Our study provides insights into the effect of overexpresion of ACEII in T. reesei.
| Original language | English |
|---|---|
| Article number | 01031 |
| Journal | Transaction on GIGAKU |
| Volume | 1 |
| Publication status | Published - 2012 |
| Externally published | Yes |