Pathogens of Dikerogammarus haemobaphes regulate host activity and survival, but also threaten native amphipod populations in the UK

Jamie Bojko, Grant Stentiford, Paul Stebbing, Chris Hassall, Alice Deacon, Benjamin Cargill, Benjamin Pile, Alison Dunn

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Abstract

ABSTRACT: Dikerogammarus haemobaphes is a non-native amphipod in UK freshwaters. Studies
have identified this species as a low-impact invader in the UK, relative to its cousin Dikero gammarus
villosus. It has been suggested that regulation by symbionts (such as Microsporidia) could explain
this difference in impact. The effect of parasitism on D. haemobaphes is largely unknown. This
was explored herein using 2 behavioural assays measuring activity and aggregation. First, D.
haemobaphes were screened histologically post-assay, identifying 2 novel viruses (D. haemo baphes
bi-facies-like virus [DhbflV], D. haemobaphes bacilliform virus [DhBV]), Cucumispora ornata
(Micro sporidia), Apicomplexa, and Digenea, which could alter host behaviour. DhBV infection
burden increased host activity, and C. ornata infection reduced host activity. Second, native invertebrates
were collected from the invasion site at Carlton Brook, UK, and tested for the presence of
C. ornata. PCR screening identified that Gammarus pulex and other native invertebrates were
positive for C. ornata. The host range of this parasite, and its impact on host survival, was additionally
explored using D. haemobaphes, D. villosus, and G. pulex in a laboratory trial. D. haemo baphes
and G. pulex became infected by C. ornata, which also lowered survival rate. D. villosus did not
become infected. A PCR protocol for DhbflV was also applied to D. haemobaphes after the survival
trial, associating this virus with decreased host survival. In conclusion, D. haemobaphes has a
complex relationship with parasites in the UK environment. C. ornata likely regulates populations
by decreasing host
Original languageEnglish
Pages (from-to)63-79
JournalDiseases of Aquatic Organisms
Early online date1 Mar 2018
Publication statusPublished - 2 Oct 2019

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Dikerogammarus
amphipod
Amphipoda
virus
pathogen
Gammarus pulex
viruses
pathogens
parasite
assay
parasites
host range
Microsporidia
assays
symbiont
parasitism
symbionts
invertebrate
survival rate
invertebrates

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Bojko, Jamie ; Stentiford, Grant ; Stebbing, Paul ; Hassall, Chris ; Deacon, Alice ; Cargill, Benjamin ; Pile, Benjamin ; Dunn, Alison. / Pathogens of Dikerogammarus haemobaphes regulate host activity and survival, but also threaten native amphipod populations in the UK. In: Diseases of Aquatic Organisms. 2019 ; pp. 63-79.
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abstract = "ABSTRACT: Dikerogammarus haemobaphes is a non-native amphipod in UK freshwaters. Studieshave identified this species as a low-impact invader in the UK, relative to its cousin Dikero gammarusvillosus. It has been suggested that regulation by symbionts (such as Microsporidia) could explainthis difference in impact. The effect of parasitism on D. haemobaphes is largely unknown. Thiswas explored herein using 2 behavioural assays measuring activity and aggregation. First, D.haemobaphes were screened histologically post-assay, identifying 2 novel viruses (D. haemo baphesbi-facies-like virus [DhbflV], D. haemobaphes bacilliform virus [DhBV]), Cucumispora ornata(Micro sporidia), Apicomplexa, and Digenea, which could alter host behaviour. DhBV infectionburden increased host activity, and C. ornata infection reduced host activity. Second, native invertebrateswere collected from the invasion site at Carlton Brook, UK, and tested for the presence ofC. ornata. PCR screening identified that Gammarus pulex and other native invertebrates werepositive for C. ornata. The host range of this parasite, and its impact on host survival, was additionallyexplored using D. haemobaphes, D. villosus, and G. pulex in a laboratory trial. D. haemo baphesand G. pulex became infected by C. ornata, which also lowered survival rate. D. villosus did notbecome infected. A PCR protocol for DhbflV was also applied to D. haemobaphes after the survivaltrial, associating this virus with decreased host survival. In conclusion, D. haemobaphes has acomplex relationship with parasites in the UK environment. C. ornata likely regulates populationsby decreasing host",
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Pathogens of Dikerogammarus haemobaphes regulate host activity and survival, but also threaten native amphipod populations in the UK. / Bojko, Jamie; Stentiford, Grant; Stebbing, Paul; Hassall, Chris; Deacon, Alice ; Cargill, Benjamin; Pile, Benjamin; Dunn, Alison.

In: Diseases of Aquatic Organisms, 02.10.2019, p. 63-79.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Pathogens of Dikerogammarus haemobaphes regulate host activity and survival, but also threaten native amphipod populations in the UK

AU - Bojko, Jamie

AU - Stentiford, Grant

AU - Stebbing, Paul

AU - Hassall, Chris

AU - Deacon, Alice

AU - Cargill, Benjamin

AU - Pile, Benjamin

AU - Dunn, Alison

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N2 - ABSTRACT: Dikerogammarus haemobaphes is a non-native amphipod in UK freshwaters. Studieshave identified this species as a low-impact invader in the UK, relative to its cousin Dikero gammarusvillosus. It has been suggested that regulation by symbionts (such as Microsporidia) could explainthis difference in impact. The effect of parasitism on D. haemobaphes is largely unknown. Thiswas explored herein using 2 behavioural assays measuring activity and aggregation. First, D.haemobaphes were screened histologically post-assay, identifying 2 novel viruses (D. haemo baphesbi-facies-like virus [DhbflV], D. haemobaphes bacilliform virus [DhBV]), Cucumispora ornata(Micro sporidia), Apicomplexa, and Digenea, which could alter host behaviour. DhBV infectionburden increased host activity, and C. ornata infection reduced host activity. Second, native invertebrateswere collected from the invasion site at Carlton Brook, UK, and tested for the presence ofC. ornata. PCR screening identified that Gammarus pulex and other native invertebrates werepositive for C. ornata. The host range of this parasite, and its impact on host survival, was additionallyexplored using D. haemobaphes, D. villosus, and G. pulex in a laboratory trial. D. haemo baphesand G. pulex became infected by C. ornata, which also lowered survival rate. D. villosus did notbecome infected. A PCR protocol for DhbflV was also applied to D. haemobaphes after the survivaltrial, associating this virus with decreased host survival. In conclusion, D. haemobaphes has acomplex relationship with parasites in the UK environment. C. ornata likely regulates populationsby decreasing host

AB - ABSTRACT: Dikerogammarus haemobaphes is a non-native amphipod in UK freshwaters. Studieshave identified this species as a low-impact invader in the UK, relative to its cousin Dikero gammarusvillosus. It has been suggested that regulation by symbionts (such as Microsporidia) could explainthis difference in impact. The effect of parasitism on D. haemobaphes is largely unknown. Thiswas explored herein using 2 behavioural assays measuring activity and aggregation. First, D.haemobaphes were screened histologically post-assay, identifying 2 novel viruses (D. haemo baphesbi-facies-like virus [DhbflV], D. haemobaphes bacilliform virus [DhBV]), Cucumispora ornata(Micro sporidia), Apicomplexa, and Digenea, which could alter host behaviour. DhBV infectionburden increased host activity, and C. ornata infection reduced host activity. Second, native invertebrateswere collected from the invasion site at Carlton Brook, UK, and tested for the presence ofC. ornata. PCR screening identified that Gammarus pulex and other native invertebrates werepositive for C. ornata. The host range of this parasite, and its impact on host survival, was additionallyexplored using D. haemobaphes, D. villosus, and G. pulex in a laboratory trial. D. haemo baphesand G. pulex became infected by C. ornata, which also lowered survival rate. D. villosus did notbecome infected. A PCR protocol for DhbflV was also applied to D. haemobaphes after the survivaltrial, associating this virus with decreased host survival. In conclusion, D. haemobaphes has acomplex relationship with parasites in the UK environment. C. ornata likely regulates populationsby decreasing host

M3 - Article

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EP - 79

JO - Diseases of Aquatic Organisms

JF - Diseases of Aquatic Organisms

SN - 0177-5103

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