Plasmonic/magnetic molybdenum trioxide and graphitic carbon nitride quantum dots-based fluoroimmunosensing system for influenza virus

Ojodomo J. Achadu; Kenshin Takemura; Indra Memdi Khoris; Enoch Y. Park

doi:10.1016/j.snb.2020.128494

Plasmonic/magnetic molybdenum trioxide and graphitic carbon nitride quantum dots-based fluoroimmunosensing system for influenza virus

Ojodomo J. Achadu, Kenshin Takemura, Indra Memdi Khoris, Enoch Y. Park

SHLS Life Sciences

Research output: Contribution to journal › Article › peer-review

27 Citations (Scopus)

Abstract

A novel magnetic/plasmonic-assisted fluoro-immunoassay system is developed for the detection of influenza virus using magnetic-derivatized plasmonic molybdenum trioxide quantum dots (MP-MoO₃ QDs) as the plasmonic/magnetic agent and fluorescent graphitic carbon nitride quantum dots (gCNQDs) as the monitoring probe. Specific antibody against influenza A virus was conjugated onto the surface of MP-MoO₃ QDs and gCNQDs, respectively. In the presence of influenza A virus (as the test virus), a core-satellite immunocomplex is formed between the antibody-conjugated nanomaterials (Ab-MP-MoO₃ QDs and Ab-gCNQDs) and their interaction resulted in the modulation and gradual enhancement of the fluorescence intensity of the detection probe with the influenza virus concentration-dependent increase. In addition, PL change without influenza A virus was not observed. Limits of detection of 0.25 and 0.9 pg/mL were achieved for Influenza virus A/New Caledonia (20/99/IVR/116) (H1N1) detection in deionized water and human serum, respectively. Clinically isolated influenza virus A/Yokohama (110/2009) (H3N2) was detected in the range of 45 – 25,000 PFU/mL, with a limit of detection ca 45 PFU/mL (as opposed to a minimum of 5000 PFU/mL for a commercial test kit). This developed biosensor provides a robust, sensitive as well as a selective platform for influenza virus detection.

Original language	English
Article number	128494
Journal	Sensors and Actuators, B: Chemical
Volume	321
Early online date	22 Jun 2020
DOIs	https://doi.org/10.1016/j.snb.2020.128494
Publication status	Published - 2 Jul 2020

Bibliographical note

Funding Information:
The authors sincerely thank Professor K. Morita of the Institute of Tropical Medicine, Nagasaki University and Professor T. Suzuki of Hamamatsu University School of Medicine for providing the Zika virus and influenza virus A/H3N2, respectively. O.J.A gratefully thanks the Japan Society for the Promotion of Science (JSPS) for a Postdoctoral Fellowship for Research in Japan (Standard) (Grant No. 19F19348).

Funding Information:
The authors sincerely thank Professor K. Morita of the Institute of Tropical Medicine, Nagasaki University and Professor T. Suzuki of Hamamatsu University School of Medicine for providing the Zika virus and influenza virus A/H3N2, respectively. O.J.A gratefully thanks the Japan Society for the Promotion of Science (JSPS) for a Postdoctoral Fellowship for Research in Japan (Standard) (Grant No. 19F19348 ).

Publisher Copyright:
© 2020 Elsevier B.V.

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title = "Plasmonic/magnetic molybdenum trioxide and graphitic carbon nitride quantum dots-based fluoroimmunosensing system for influenza virus",

abstract = "A novel magnetic/plasmonic-assisted fluoro-immunoassay system is developed for the detection of influenza virus using magnetic-derivatized plasmonic molybdenum trioxide quantum dots (MP-MoO3 QDs) as the plasmonic/magnetic agent and fluorescent graphitic carbon nitride quantum dots (gCNQDs) as the monitoring probe. Specific antibody against influenza A virus was conjugated onto the surface of MP-MoO3 QDs and gCNQDs, respectively. In the presence of influenza A virus (as the test virus), a core-satellite immunocomplex is formed between the antibody-conjugated nanomaterials (Ab-MP-MoO3 QDs and Ab-gCNQDs) and their interaction resulted in the modulation and gradual enhancement of the fluorescence intensity of the detection probe with the influenza virus concentration-dependent increase. In addition, PL change without influenza A virus was not observed. Limits of detection of 0.25 and 0.9 pg/mL were achieved for Influenza virus A/New Caledonia (20/99/IVR/116) (H1N1) detection in deionized water and human serum, respectively. Clinically isolated influenza virus A/Yokohama (110/2009) (H3N2) was detected in the range of 45 – 25,000 PFU/mL, with a limit of detection ca 45 PFU/mL (as opposed to a minimum of 5000 PFU/mL for a commercial test kit). This developed biosensor provides a robust, sensitive as well as a selective platform for influenza virus detection.",

author = "Achadu, {Ojodomo J.} and Kenshin Takemura and Khoris, {Indra Memdi} and Park, {Enoch Y.}",

note = "Funding Information: The authors sincerely thank Professor K. Morita of the Institute of Tropical Medicine, Nagasaki University and Professor T. Suzuki of Hamamatsu University School of Medicine for providing the Zika virus and influenza virus A/H3N2, respectively. O.J.A gratefully thanks the Japan Society for the Promotion of Science (JSPS) for a Postdoctoral Fellowship for Research in Japan (Standard) (Grant No. 19F19348). Funding Information: The authors sincerely thank Professor K. Morita of the Institute of Tropical Medicine, Nagasaki University and Professor T. Suzuki of Hamamatsu University School of Medicine for providing the Zika virus and influenza virus A/H3N2, respectively. O.J.A gratefully thanks the Japan Society for the Promotion of Science (JSPS) for a Postdoctoral Fellowship for Research in Japan (Standard) (Grant No. 19F19348 ). Publisher Copyright: {\textcopyright} 2020 Elsevier B.V.",

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AU - Khoris, Indra Memdi

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N1 - Funding Information: The authors sincerely thank Professor K. Morita of the Institute of Tropical Medicine, Nagasaki University and Professor T. Suzuki of Hamamatsu University School of Medicine for providing the Zika virus and influenza virus A/H3N2, respectively. O.J.A gratefully thanks the Japan Society for the Promotion of Science (JSPS) for a Postdoctoral Fellowship for Research in Japan (Standard) (Grant No. 19F19348). Funding Information: The authors sincerely thank Professor K. Morita of the Institute of Tropical Medicine, Nagasaki University and Professor T. Suzuki of Hamamatsu University School of Medicine for providing the Zika virus and influenza virus A/H3N2, respectively. O.J.A gratefully thanks the Japan Society for the Promotion of Science (JSPS) for a Postdoctoral Fellowship for Research in Japan (Standard) (Grant No. 19F19348 ). Publisher Copyright: © 2020 Elsevier B.V.

PY - 2020/7/2

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N2 - A novel magnetic/plasmonic-assisted fluoro-immunoassay system is developed for the detection of influenza virus using magnetic-derivatized plasmonic molybdenum trioxide quantum dots (MP-MoO3 QDs) as the plasmonic/magnetic agent and fluorescent graphitic carbon nitride quantum dots (gCNQDs) as the monitoring probe. Specific antibody against influenza A virus was conjugated onto the surface of MP-MoO3 QDs and gCNQDs, respectively. In the presence of influenza A virus (as the test virus), a core-satellite immunocomplex is formed between the antibody-conjugated nanomaterials (Ab-MP-MoO3 QDs and Ab-gCNQDs) and their interaction resulted in the modulation and gradual enhancement of the fluorescence intensity of the detection probe with the influenza virus concentration-dependent increase. In addition, PL change without influenza A virus was not observed. Limits of detection of 0.25 and 0.9 pg/mL were achieved for Influenza virus A/New Caledonia (20/99/IVR/116) (H1N1) detection in deionized water and human serum, respectively. Clinically isolated influenza virus A/Yokohama (110/2009) (H3N2) was detected in the range of 45 – 25,000 PFU/mL, with a limit of detection ca 45 PFU/mL (as opposed to a minimum of 5000 PFU/mL for a commercial test kit). This developed biosensor provides a robust, sensitive as well as a selective platform for influenza virus detection.

AB - A novel magnetic/plasmonic-assisted fluoro-immunoassay system is developed for the detection of influenza virus using magnetic-derivatized plasmonic molybdenum trioxide quantum dots (MP-MoO3 QDs) as the plasmonic/magnetic agent and fluorescent graphitic carbon nitride quantum dots (gCNQDs) as the monitoring probe. Specific antibody against influenza A virus was conjugated onto the surface of MP-MoO3 QDs and gCNQDs, respectively. In the presence of influenza A virus (as the test virus), a core-satellite immunocomplex is formed between the antibody-conjugated nanomaterials (Ab-MP-MoO3 QDs and Ab-gCNQDs) and their interaction resulted in the modulation and gradual enhancement of the fluorescence intensity of the detection probe with the influenza virus concentration-dependent increase. In addition, PL change without influenza A virus was not observed. Limits of detection of 0.25 and 0.9 pg/mL were achieved for Influenza virus A/New Caledonia (20/99/IVR/116) (H1N1) detection in deionized water and human serum, respectively. Clinically isolated influenza virus A/Yokohama (110/2009) (H3N2) was detected in the range of 45 – 25,000 PFU/mL, with a limit of detection ca 45 PFU/mL (as opposed to a minimum of 5000 PFU/mL for a commercial test kit). This developed biosensor provides a robust, sensitive as well as a selective platform for influenza virus detection.

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Plasmonic/magnetic molybdenum trioxide and graphitic carbon nitride quantum dots-based fluoroimmunosensing system for influenza virus

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