Potassium uptake and retention by Oceanomonas baumannii at low water activity in the presence of phenol

Geoffrey R. Brown, Stephen P. Cummings

Research output: Contribution to journalArticleResearchpeer-review

7 Citations (Scopus)

Abstract

Oceanomonas baumanniiT (ATCC 700832) is a halotolerant bacterium capable of degrading phenol, which requires potassium in order for turgor growth to occur in minimal medium containing 5% NaCl (w/v). However, at this salinity growth can be inhibited by reduced potassium concentrations. The affinity for potassium (KS) was determined to be 219 μM and 408 μM for cultures utilising phenol and succinate respectively as the sole carbon source for growth. Rubidium but not caesium could substitute for potassium in alleviating growth inhibition due to potassium limitation. The effect of elevated phenol on potassium retention was studied, and it was shown that contrary to expectations, as external phenol concentration was increased the levels of intracellular potassium were significantly elevated. This observation correlated with changes in the cytoplasmic membrane, particularly the increase in the saturated:unsaturated fatty acid ratio from 0.47 to 1.44, and the decrease in the zwitterionic:anionic phospholipid ratio from 2.23 to 1.22. Both these changes promote membrane bilayer configurations and increase lipid ordering of the membrane reducing its permeability and inhibiting cation efflux.

Original languageEnglish
Pages (from-to)37-41
Number of pages5
JournalFEMS Microbiology Letters
Volume205
Issue number1
DOIs
Publication statusPublished - 27 Nov 2001

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Phenol
Potassium
Water
Growth
Rubidium
Cesium
Salinity
Succinic Acid
Membrane Lipids
Unsaturated Fatty Acids
Cations
Permeability
Phospholipids
Fatty Acids
Carbon
Cell Membrane
Bacteria
Membranes

Cite this

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abstract = "Oceanomonas baumanniiT (ATCC 700832) is a halotolerant bacterium capable of degrading phenol, which requires potassium in order for turgor growth to occur in minimal medium containing 5{\%} NaCl (w/v). However, at this salinity growth can be inhibited by reduced potassium concentrations. The affinity for potassium (KS) was determined to be 219 μM and 408 μM for cultures utilising phenol and succinate respectively as the sole carbon source for growth. Rubidium but not caesium could substitute for potassium in alleviating growth inhibition due to potassium limitation. The effect of elevated phenol on potassium retention was studied, and it was shown that contrary to expectations, as external phenol concentration was increased the levels of intracellular potassium were significantly elevated. This observation correlated with changes in the cytoplasmic membrane, particularly the increase in the saturated:unsaturated fatty acid ratio from 0.47 to 1.44, and the decrease in the zwitterionic:anionic phospholipid ratio from 2.23 to 1.22. Both these changes promote membrane bilayer configurations and increase lipid ordering of the membrane reducing its permeability and inhibiting cation efflux.",
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Potassium uptake and retention by Oceanomonas baumannii at low water activity in the presence of phenol. / Brown, Geoffrey R.; Cummings, Stephen P.

In: FEMS Microbiology Letters, Vol. 205, No. 1, 27.11.2001, p. 37-41.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

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AU - Brown, Geoffrey R.

AU - Cummings, Stephen P.

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AB - Oceanomonas baumanniiT (ATCC 700832) is a halotolerant bacterium capable of degrading phenol, which requires potassium in order for turgor growth to occur in minimal medium containing 5% NaCl (w/v). However, at this salinity growth can be inhibited by reduced potassium concentrations. The affinity for potassium (KS) was determined to be 219 μM and 408 μM for cultures utilising phenol and succinate respectively as the sole carbon source for growth. Rubidium but not caesium could substitute for potassium in alleviating growth inhibition due to potassium limitation. The effect of elevated phenol on potassium retention was studied, and it was shown that contrary to expectations, as external phenol concentration was increased the levels of intracellular potassium were significantly elevated. This observation correlated with changes in the cytoplasmic membrane, particularly the increase in the saturated:unsaturated fatty acid ratio from 0.47 to 1.44, and the decrease in the zwitterionic:anionic phospholipid ratio from 2.23 to 1.22. Both these changes promote membrane bilayer configurations and increase lipid ordering of the membrane reducing its permeability and inhibiting cation efflux.

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