Prospecting for new bacterial metabolites: a glossary of approaches for inducing, activating and upregulating the biosynthesis of bacterial cryptic or silent natural products

Joseph Scott Zarins-Tutt, Tania Triscari Barberi, Hong Gao, Andrew Mearns-Spragg, Lixin Zhang, David J Newman, Rebecca Jane Miriam Goss

Research output: Contribution to journalReview article

Abstract

Covering: up to 2015. Over the centuries, microbial secondary metabolites have played a central role in the treatment of human diseases and have revolutionised the pharmaceutical industry. With the increasing number of sequenced microbial genomes revealing a plethora of novel biosynthetic genes, natural product drug discovery is entering an exciting second golden age. Here, we provide a concise overview as an introductory guide to the main methods employed to unlock or up-regulate these so called 'cryptic', 'silent' and 'orphan' gene clusters, and increase the production of the encoded natural product. With a predominant focus on bacterial natural products we will discuss the importance of the bioinformatics approach for genome mining, the use of first different and simple culturing techniques and then the application of genetic engineering to unlock the microbial treasure trove.

Original languageEnglish
Pages (from-to)54-72
Number of pages19
JournalNatural Product Reports
Volume33
Issue number1
DOIs
Publication statusPublished - Jan 2016

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Biosynthesis
Glossaries
Metabolites
Biological Products
Genes
Microbial Genome
Orphaned Children
Genetic Engineering
Drug Industry
Drug Discovery
Multigene Family
Computational Biology
Genetic engineering
Up-Regulation
Bioinformatics
Genome
Pharmaceutical Preparations
Industry
Therapeutics

Cite this

Zarins-Tutt, Joseph Scott ; Barberi, Tania Triscari ; Gao, Hong ; Mearns-Spragg, Andrew ; Zhang, Lixin ; Newman, David J ; Goss, Rebecca Jane Miriam. / Prospecting for new bacterial metabolites : a glossary of approaches for inducing, activating and upregulating the biosynthesis of bacterial cryptic or silent natural products. In: Natural Product Reports. 2016 ; Vol. 33, No. 1. pp. 54-72.
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Prospecting for new bacterial metabolites : a glossary of approaches for inducing, activating and upregulating the biosynthesis of bacterial cryptic or silent natural products. / Zarins-Tutt, Joseph Scott; Barberi, Tania Triscari; Gao, Hong; Mearns-Spragg, Andrew; Zhang, Lixin; Newman, David J; Goss, Rebecca Jane Miriam.

In: Natural Product Reports, Vol. 33, No. 1, 01.2016, p. 54-72.

Research output: Contribution to journalReview article

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