Prospecting for new bacterial metabolites: a glossary of approaches for inducing, activating and upregulating the biosynthesis of bacterial cryptic or silent natural products

Joseph Scott Zarins-Tutt, Tania Triscari Barberi, Hong Gao, Andrew Mearns-Spragg, Lixin Zhang, David J Newman, Rebecca Jane Miriam Goss

    Research output: Contribution to journalReview article

    Abstract

    Covering: up to 2015. Over the centuries, microbial secondary metabolites have played a central role in the treatment of human diseases and have revolutionised the pharmaceutical industry. With the increasing number of sequenced microbial genomes revealing a plethora of novel biosynthetic genes, natural product drug discovery is entering an exciting second golden age. Here, we provide a concise overview as an introductory guide to the main methods employed to unlock or up-regulate these so called 'cryptic', 'silent' and 'orphan' gene clusters, and increase the production of the encoded natural product. With a predominant focus on bacterial natural products we will discuss the importance of the bioinformatics approach for genome mining, the use of first different and simple culturing techniques and then the application of genetic engineering to unlock the microbial treasure trove.

    Original languageEnglish
    Pages (from-to)54-72
    Number of pages19
    JournalNatural Product Reports
    Volume33
    Issue number1
    DOIs
    Publication statusPublished - Jan 2016

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