Regulation of cerebral microvascular permeability by histamine in the anaesthetized rat

Mosharraf Sarker, A. S. Easton, P. A. Fraser

Research output: Contribution to journalArticleResearchpeer-review

38 Citations (Scopus)

Abstract

1. The permeability response of slightly leaky pial venular capillaries to histamine was investigated using the single microvessel occlusion technique. 2. Histamine dose-response curves showed that concentrations between 5 nM and 5 μM increased permeability, while concentrations from 50 μM to 5 mM reduced it. 3. The H2 receptor antagonist cimetidine (2 μM) blocked the effects of lower concentrations of histamine, while the H1 receptor antagonist mepyramine (3 nM) blocked those of higher concentrations of histamine. 4. The effects of lower doses of histamine were mimicked by the H2 receptor agonist dimaprit, and the effects of higher doses of histamine were mimicked by the H1 receptor agonist α-2-(2-aminoethyl)pyridine (AEP). 5. Low concentrations of histamine, which normally increase the permeability of Lucifer Yellow (P(LY)), reduced it when co-applied with the phosphodiesterase 4 (PDE4) inhibitor rolipram. Rolipram also potentiated the response to AEP, but had no effect on that to dimaprit. 6. The effects of dimaprit were blocked by reducing extracellular Ca2+ from 2.5 mM to nominally Ca2+ free, or by applying the calcium entry blocker SKF 96365.

Original languageEnglish
Pages (from-to)909-918
Number of pages10
JournalJournal of Physiology
Volume507
Issue number3
DOIs
Publication statusPublished - 15 Mar 1998

Fingerprint

Capillary Permeability
Histamine
Dimaprit
Histamine Agonists
Rolipram
Permeability
Histamine H2 Receptors
1-(2-(3-(4-methoxyphenyl)propoxy)-4-methoxyphenylethyl)-1H-imidazole
Phosphodiesterase 4 Inhibitors
Histamine H1 Antagonists
Pyrilamine
Histamine H1 Receptors
Cimetidine
Microvessels
Calcium

Cite this

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abstract = "1. The permeability response of slightly leaky pial venular capillaries to histamine was investigated using the single microvessel occlusion technique. 2. Histamine dose-response curves showed that concentrations between 5 nM and 5 μM increased permeability, while concentrations from 50 μM to 5 mM reduced it. 3. The H2 receptor antagonist cimetidine (2 μM) blocked the effects of lower concentrations of histamine, while the H1 receptor antagonist mepyramine (3 nM) blocked those of higher concentrations of histamine. 4. The effects of lower doses of histamine were mimicked by the H2 receptor agonist dimaprit, and the effects of higher doses of histamine were mimicked by the H1 receptor agonist α-2-(2-aminoethyl)pyridine (AEP). 5. Low concentrations of histamine, which normally increase the permeability of Lucifer Yellow (P(LY)), reduced it when co-applied with the phosphodiesterase 4 (PDE4) inhibitor rolipram. Rolipram also potentiated the response to AEP, but had no effect on that to dimaprit. 6. The effects of dimaprit were blocked by reducing extracellular Ca2+ from 2.5 mM to nominally Ca2+ free, or by applying the calcium entry blocker SKF 96365.",
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Regulation of cerebral microvascular permeability by histamine in the anaesthetized rat. / Sarker, Mosharraf; Easton, A. S.; Fraser, P. A.

In: Journal of Physiology, Vol. 507, No. 3, 15.03.1998, p. 909-918.

Research output: Contribution to journalArticleResearchpeer-review

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