TY - JOUR
T1 - Rhamnolipid biosurfactant production by strains of Pseudomonas aeruginosa using low-cost raw materials
AU - Rahman, Pattanathu
AU - Rahman, Thahira J.
AU - McClean, Stephen
AU - Marchant, Roger
AU - Banat, Ibrahim M.
N1 - subject to Restrictions below, author can archive publisher's version/PDF. For full details see http://www.sherpa.ac.uk/romeo/ [Accessed 15/11/2010]
PY - 2002/9/1
Y1 - 2002/9/1
N2 - This study was aimed at the development of economical methods for higher yields of biosurfactant by suggesting the use of low-cost raw materials. Two oil-degrading strains, Pseudomonas aeruginosa GS9-119 and DS10-129, were used to optimize a substrate for maximum rhamnolipid production. Among the two strains, the latter produced maxima of 4.31, 2.98, and 1.77 g/L rhamnolipid biosurfactant using soybean oil, safflower oil, and glycerol, respectively. The yield of biosurfactant steadily increased even after the bacterial cultures reached the stationary phase of growth. Characterization of rhamnolipids using mass spectrometry revealed the presence of dirhamnolipids (Rha-Rha-C10-C10). Emulsification activity of the rhamnolipid biosurfactant produced by P. aeruginosa DS10-129 was greater than 70% using all the hydrocarbons tested, including xylene, benzene, hexane, crude oil, kerosene, gasoline, and diesel. P. aeruginosa GS9-119 emulsified only hexane and kerosene to that level.
AB - This study was aimed at the development of economical methods for higher yields of biosurfactant by suggesting the use of low-cost raw materials. Two oil-degrading strains, Pseudomonas aeruginosa GS9-119 and DS10-129, were used to optimize a substrate for maximum rhamnolipid production. Among the two strains, the latter produced maxima of 4.31, 2.98, and 1.77 g/L rhamnolipid biosurfactant using soybean oil, safflower oil, and glycerol, respectively. The yield of biosurfactant steadily increased even after the bacterial cultures reached the stationary phase of growth. Characterization of rhamnolipids using mass spectrometry revealed the presence of dirhamnolipids (Rha-Rha-C10-C10). Emulsification activity of the rhamnolipid biosurfactant produced by P. aeruginosa DS10-129 was greater than 70% using all the hydrocarbons tested, including xylene, benzene, hexane, crude oil, kerosene, gasoline, and diesel. P. aeruginosa GS9-119 emulsified only hexane and kerosene to that level.
UR - http://www.scopus.com/inward/record.url?scp=0036860709&partnerID=8YFLogxK
U2 - 10.1021/bp020071x
DO - 10.1021/bp020071x
M3 - Article
C2 - 12467462
AN - SCOPUS:0036860709
SN - 8756-7938
VL - 18
SP - 1277
EP - 1281
JO - Biotechnology Progress
JF - Biotechnology Progress
IS - 6
ER -