Roles of sodium hydrosulfide and sodium nitroprusside as priming molecules during drought acclimation in citrus plants

Vasileios Ziogas, Georgia Tanou, Maya Belghazi, Panagiota Filippou, Vasileios Fotopoulos, Diamantidis Grigorios, Athanassios Molassiotis

    Research output: Contribution to journalArticle

    31 Citations (Scopus)

    Abstract

    Emerging evidence suggests that the gaseous molecules hydrogen sulfide (H2S) and nitric oxide (NO) enhances plant acclimation to stress; however, the underlying mechanism remains unclear. In this work, we explored if pretreatment of citrus roots with NaHS (a H2S donor) or sodium nitroprusside (SNP, a NO donor) for 2 days (d) could elicit long-lasting priming effects to subsequent exposure to PEG-associated drought stress for 21 d following a 5 d acclimation period. Detailed physiological study documented that both pretreatments primed plants against drought stress. Analysis of the level of nitrite, NOx, S-nitrosoglutahione reductase, Tyr-nitration and S-nitrosylation along with the expression of genes involved in NO-generation suggested that the nitrosative status of leaves and roots was altered by NaHS and SNP. Using a proteomic approach we characterized S-nitrosylated proteins in citrus leaves exposed to chemical treatments, including well known and novel S-nitrosylated targets. Mass spectrometry analysis also enabled the identification of 42 differentially expressed proteins in PEG alone-treated plants. Several PEG-responsive proteins were down-regulated, especially photosynthetic proteins. Finally, the identification of specific proteins that were regulated by NaHS and SNP under PEG conditions provides novel insight into long-term drought priming in plants and in a fruit crop such as citrus in particular.

    Original languageEnglish
    Pages (from-to)433-450
    Number of pages18
    JournalPlant Molecular Biology
    Volume89
    Issue number4-5
    DOIs
    Publication statusPublished - 24 Sep 2015

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