S-selective hydroxynitrile lyase from a plant Baliospermum montanum: Molecular characterization of recombinant enzyme

Keyphrases

• Molecular Characterization 100%
• Recombinant Enzyme 100%
• Hydroxynitrile Lyase 100%
• Baliospermum Montanum 100%
• Kcat 100%
• Escherichia Coli 33%
• High Activity 33%
• Enzyme Production 33%
• Kinetic Study 33%
• Genomic DNA (gDNA) 33%
• Molecular Mass 33%
• Truncated Forms 33%
• Silver Nitrate 33%
• Column Chromatography 33%
• Truncation 33%
• Dimeric Structure 33%
• Entrance Channel 33%
• Aromatic Aldehydes 33%
• Aliphatic Aldehydes 33%
• Buffer Composition 33%
• Cyanohydrin Synthesis 33%
• Wide pH Range 33%
• Ammonium Sulfate Fractionation 33%
• Aliphatic Ketones 33%
• Piperonal 33%
• Full-length cDNA 33%
• Detailed Chemical Kinetics 33%
• Substrate Pocket 33%
• Gel Filtration 33%
• Methyl Ethyl Ketone 33%
• High Specific Activity 33%
• Protein Subunit 33%

Chemistry

• Kcat/Km 100%
• Aliphatic Aldehyde 50%
• Ammonium Sulfate 50%
• Size Exclusion Chromatography 50%
• Kcat 50%
• Piperonal 50%