Second-harmonic generation imaging of collagen in ancient bone

B Thomas, D McKintosh, T Fildes, L Smith, F Hargrave, Meez Islam, Timothy Thompson, R Layfield, D Scott, B Shaw, C. L Burrell, S Gonzalez, S Taylor

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Abstract

Second-harmonic generation imaging (SHG) captures triple helical collagen molecules near tissue surfaces. Biomedical research routinely utilizes various imaging software packages to quantify SHG signals for collagen content and distribution estimates in modern tissue samples including bone. For the first time using SHG, samples of modern, medieval, and ice age bones were imaged to test the applicability of SHG to ancient bone from a variety of ages, settings, and taxa. Four independent techniques including Raman spectroscopy, FTIR spectroscopy, radiocarbon dating protocols, and mass spectrometry-based protein sequencing, confirm the presence of protein, consistent with the hypothesis that SHG imaging detects ancient bone collagen. These results suggest that future studies have the potential to use SHG imaging to provide new insights into the composition of ancient bone, to characterize ancient bone disorders, to investigate collagen preservation within and between various taxa, and to monitor collagen decay regimes in different depositional environments.
Original languageEnglish
Pages (from-to)-
JournalBone Reports
DOIs
Publication statusPublished - 1 Nov 2017

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Collagen
Bone and Bones
Radiometric Dating
Raman Spectrum Analysis
Protein Sequence Analysis
Ice
Fourier Transform Infrared Spectroscopy
Biomedical Research
Mass Spectrometry
Spectrum Analysis
Software
Proteins

Bibliographical note

Confirmed by author email as Gold open access [20.03.18] For full details see https://www.elsevier.com/about/open-science/open-access

Cite this

Thomas, B., McKintosh, D., Fildes, T., Smith, L., Hargrave, F., Islam, M., ... Taylor, S. (2017). Second-harmonic generation imaging of collagen in ancient bone. Bone Reports, -. https://doi.org/10.1016/j.bonr.2017.10.005
Thomas, B ; McKintosh, D ; Fildes, T ; Smith, L ; Hargrave, F ; Islam, Meez ; Thompson, Timothy ; Layfield, R ; Scott, D ; Shaw, B ; Burrell, C. L ; Gonzalez, S ; Taylor, S. / Second-harmonic generation imaging of collagen in ancient bone. In: Bone Reports. 2017 ; pp. -.
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Thomas, B, McKintosh, D, Fildes, T, Smith, L, Hargrave, F, Islam, M, Thompson, T, Layfield, R, Scott, D, Shaw, B, Burrell, CL, Gonzalez, S & Taylor, S 2017, 'Second-harmonic generation imaging of collagen in ancient bone', Bone Reports, pp. -. https://doi.org/10.1016/j.bonr.2017.10.005

Second-harmonic generation imaging of collagen in ancient bone. / Thomas, B; McKintosh, D; Fildes, T; Smith, L; Hargrave, F; Islam, Meez; Thompson, Timothy; Layfield, R; Scott, D; Shaw, B; Burrell, C. L; Gonzalez, S; Taylor, S.

In: Bone Reports, 01.11.2017, p. -.

Research output: Contribution to journalArticleResearchpeer-review

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AU - Thompson, Timothy

AU - Layfield, R

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AU - Shaw, B

AU - Burrell, C. L

AU - Gonzalez, S

AU - Taylor, S

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