TY - JOUR
T1 - Single nucleotide polymorphism analysis of a trichoderma reesei hyper-cellulolytic mutant developed in japan
AU - De Oliveira Porciuncula, Juliano
AU - Furukawa, Takanori
AU - Mori, Kazuki
AU - Shida, Yosuke
AU - Hirakawa, Hideki
AU - Tashiro, Kosuke
AU - Kuhara, Satoru
AU - Nakagawa, Satoshi
AU - Morikawa, Yasushi
AU - Ogasawara, Wataru
PY - 2013/3/7
Y1 - 2013/3/7
N2 - The ascomycete Trichoderma reesei is known as one of the most prolific producers of plant biomass-degrading enzymes. While several mutant strains have been developed by mutagenesis to improve enzyme productivity for a variety of industrial applications, little is known about the mechanical basis of these improvements. A genomic sequence comparison of mutant and wild-type strains was undertaken to provide new insights in this regard. We identified a number of singlenucleotide polymorphisms (SNPs) after sequencing the genome of a hyper-cellulolytic T. reesei strain, PC-3-7, with a next-generation sequencer. Of these, the SNP detected in cre1, the carbon catabolite repressor gene, was found to be responsible for increased cellulase production. Further comparative genomic analysis enabled the identification of an SNP that correlated well with high cellulase production in a T. reesei mutant. These results provide a better understanding of the genetic changes induced by classical mutagenesis and how they correlate with desirable phenotypes in filamentous fungi.
AB - The ascomycete Trichoderma reesei is known as one of the most prolific producers of plant biomass-degrading enzymes. While several mutant strains have been developed by mutagenesis to improve enzyme productivity for a variety of industrial applications, little is known about the mechanical basis of these improvements. A genomic sequence comparison of mutant and wild-type strains was undertaken to provide new insights in this regard. We identified a number of singlenucleotide polymorphisms (SNPs) after sequencing the genome of a hyper-cellulolytic T. reesei strain, PC-3-7, with a next-generation sequencer. Of these, the SNP detected in cre1, the carbon catabolite repressor gene, was found to be responsible for increased cellulase production. Further comparative genomic analysis enabled the identification of an SNP that correlated well with high cellulase production in a T. reesei mutant. These results provide a better understanding of the genetic changes induced by classical mutagenesis and how they correlate with desirable phenotypes in filamentous fungi.
UR - http://www.scopus.com/inward/record.url?scp=84876377014&partnerID=8YFLogxK
U2 - 10.1271/bbb.120794
DO - 10.1271/bbb.120794
M3 - Article
C2 - 23470758
AN - SCOPUS:84876377014
SN - 0916-8451
VL - 77
SP - 534
EP - 543
JO - Bioscience, Biotechnology and Biochemistry
JF - Bioscience, Biotechnology and Biochemistry
IS - 3
ER -