Stabilization of chromium by reductase enzyme treatment

Pattanathu Rahman, M. A.V. Murthy

    Research output: Chapter in Book/Report/Conference proceedingConference contribution

    1 Citation (Scopus)
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    Abstract

    Hexavalent chromium (Cr(VI)) is highly toxic, and a major heavy metal contaminant in the environment. An important strategy for bioremediating Cr(VI) is to microbiologically reduce it to less toxic Cr(III). One of the major routes of bacterial chromate reduction is enzymatic reduction mediated by chromate reductase after its uptake inside the cell. While the enzymatic pathway is common in aerobic bacteria, it probably also occurs anaerobically. To gain more insight about this pathway, we investigated the reduction of Cr(VI) by a highly resistant Bacillus species. Reduction depended on which of three organic substrates was used, i.e., glucose, lactate and acetate at 0.1 to 2.0 mM, and the rate of reduction decreased with increasing concentration of chromate. Because sulfhydryl sites are known to be active sites for enzyme reductase, bacterial growth and reduction of Cr(VI) by Bacillus in the presence of varying concentrations of sulfate and thiosulfate were investigated. While changes in sulfate did not affect the reduction rate, raising the thiosulfate concentration in the medium from 0.05 to 1.0 mM markedly increased the reduction rate. Thiosulfate enhanced the reduction of Cr(VI), probably by accelerating the biosynthesis of chromate reductase enzymes, although other mechanisms may be involved. Our results show that the enzymes or other substances mediating the reduction reside mostly in the cytoplasm. This reductase enzyme could be extracted and applied sites contaminated with Cr(VI), to convert it to Cr(III), which would prevent leaching of the pollutant to groundwater.

    Original languageEnglish
    Title of host publicationStabilisation/Solidification Treatment and Remediation
    Subtitle of host publicationAdvances in S/S for Waste and Contaminated Land - Proceedings of the Int. Conf. on Stabilisation/Solidification Treatment and Remediation
    Pages347-355
    Publication statusPublished - 1 Dec 2005
    EventInternational Conference on Stabilisation/Solidification Treatment and Remediation: Advances in S/S for Waste and Contaminated Land - Cambridge, United Kingdom
    Duration: 12 Apr 200513 Apr 2005

    Conference

    ConferenceInternational Conference on Stabilisation/Solidification Treatment and Remediation
    CountryUnited Kingdom
    CityCambridge
    Period12/04/0513/04/05

    Bibliographical note

    'Our general policy is to allow chapter inclusion in open-access repositories provided the material is more than 18-months old'. [Email from Taylor and Francis].

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  • Cite this

    Rahman, P., & Murthy, M. A. V. (2005). Stabilization of chromium by reductase enzyme treatment. In Stabilisation/Solidification Treatment and Remediation: Advances in S/S for Waste and Contaminated Land - Proceedings of the Int. Conf. on Stabilisation/Solidification Treatment and Remediation (pp. 347-355)