Statistical optimization for lipase production from solid waste of vegetable oil industry

Rajesh Kumar Sahoo; Mohit Kumar; Swati Mohanty; Matthew Sawyer; Pattanathu  Rahman; Lala Behari Sukla; Enketeswara Subudhi

doi:10.1080/10826068.2018.1431785

Statistical optimization for lipase production from solid waste of vegetable oil industry

Rajesh Kumar Sahoo, Mohit Kumar, Swati Mohanty, Matthew Sawyer, Pattanathu Rahman, Lala Behari Sukla, Enketeswara Subudhi

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Abstract

The production of biofuel using thermostable bacterial lipase from hot spring bacteria out of low-cost agricultural residue olive oil cake is reported in the present paper. Using a lipase enzyme from Bacillus licheniformis, a 66.5% yield of methyl esters was obtained. Optimum parameters were determined, with maximum production of lipase at a pH of 8.2, temperature 50.8°C, moisture content of 55.7%, and biosurfactant content of 1.693 mg. The contour plots and 3D surface responses depict the significant interaction of pH and moisture content with biosurfactant during lipase production. Chromatographic analysis of the lipase transesterification product was methyl esters, from kitchen waste oil under optimized conditions, generated methyl palmitate, methyl stearate, methyl oleate, and methyl linoleate.

Original language	English
Pages (from-to)	321-326
Journal	Preparative Biochemistry and Biotechnology
Volume	48
Issue number	4
Early online date	9 Feb 2018
DOIs	https://doi.org/10.1080/10826068.2018.1431785
Publication status	Published - 30 Mar 2018

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abstract = "The production of biofuel using thermostable bacterial lipase from hot spring bacteria out of low-cost agricultural residue olive oil cake is reported in the present paper. Using a lipase enzyme from Bacillus licheniformis, a 66.5% yield of methyl esters was obtained. Optimum parameters were determined, with maximum production of lipase at a pH of 8.2, temperature 50.8°C, moisture content of 55.7%, and biosurfactant content of 1.693 mg. The contour plots and 3D surface responses depict the significant interaction of pH and moisture content with biosurfactant during lipase production. Chromatographic analysis of the lipase transesterification product was methyl esters, from kitchen waste oil under optimized conditions, generated methyl palmitate, methyl stearate, methyl oleate, and methyl linoleate.",

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AU - Sahoo, Rajesh Kumar

AU - Kumar, Mohit

AU - Mohanty, Swati

AU - Sawyer, Matthew

AU - Rahman, Pattanathu

AU - Sukla, Lala Behari

AU - Subudhi, Enketeswara

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AB - The production of biofuel using thermostable bacterial lipase from hot spring bacteria out of low-cost agricultural residue olive oil cake is reported in the present paper. Using a lipase enzyme from Bacillus licheniformis, a 66.5% yield of methyl esters was obtained. Optimum parameters were determined, with maximum production of lipase at a pH of 8.2, temperature 50.8°C, moisture content of 55.7%, and biosurfactant content of 1.693 mg. The contour plots and 3D surface responses depict the significant interaction of pH and moisture content with biosurfactant during lipase production. Chromatographic analysis of the lipase transesterification product was methyl esters, from kitchen waste oil under optimized conditions, generated methyl palmitate, methyl stearate, methyl oleate, and methyl linoleate.

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Statistical optimization for lipase production from solid waste of vegetable oil industry

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