Statistical optimization for lipase production from solid waste of vegetable oil industry

Rajesh Kumar Sahoo, Mohit Kumar, Swati Mohanty, Matthew Sawyer, Pattanathu Rahman, Lala Behari Sukla, Enketeswara Subudhi

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    Abstract

    The production of biofuel using thermostable bacterial lipase from hot spring bacteria out of low-cost agricultural residue olive oil cake is reported in the present paper. Using a lipase enzyme from Bacillus licheniformis, a 66.5% yield of methyl esters was obtained. Optimum parameters were determined, with maximum production of lipase at a pH of 8.2, temperature 50.8°C, moisture content of 55.7%, and biosurfactant content of 1.693 mg. The contour plots and 3D surface responses depict the significant interaction of pH and moisture content with biosurfactant during lipase production. Chromatographic analysis of the lipase transesterification product was methyl esters, from kitchen waste oil under optimized conditions, generated methyl palmitate, methyl stearate, methyl oleate, and methyl linoleate.
    Original languageEnglish
    Pages (from-to)321-326
    JournalPreparative Biochemistry and Biotechnology
    Volume48
    Issue number4
    Early online date9 Feb 2018
    DOIs
    Publication statusPublished - 30 Mar 2018

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