Candida tropicalis strain Fl0-1 and its recombinant strain CZ-15 can be used for 1,11-dicarboxylic acid (DCA13) production, and in CZ-15, one copy of carnitine acetyltransferase(CAT) genes is disrupted. The sucrose-added fed-batch fermentation of F10-1 and CZ-15 was studied. The results showed that the optimal feeding time and the optimal sucrose concentration were different for F10-1 and CZ-15. By feeding sucrose in the acid production stage, for F10-1 and CZ-15, the acid production was increased by 25.0% and 23.4%, reaching 99.5 and 108.1 g / L, and the alkane conversion rate was increased by 35.5% and 28.6%, respectively.
|Original language||Chinese (Simplified)|
|Number of pages||4|
|Journal||Guocheng Gongcheng Xuebao/The Chinese Journal of Process Engineering|
|Publication status||Published - 1 Oct 2006|