Sulfur-doped carbon dots@polydopamine-functionalized magnetic silver nanocubes for dual-modality detection of norovirus

Ojodomo J. Achadu, Fuyuki Abe, Farzana Hossain, Fahmida Nasrin, Masahito Yamazaki, Tetsuro Suzuki, Enoch Y. Park

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11 Citations (Scopus)


Synergistic dual-mode optical platforms are up-and-coming detection tools in the diagnosis and management of infectious diseases. Here, novel dual-modality fluorescence (FL) and surface-enhanced Raman scattering (SERS) techniques have been integrated into a single probe for the rapid and ultrasensitive detection of norovirus (NoV). The developed FL-SER-based biosensor relies on the dual-signal enhancements of newly synthesized sulfur-doped agar-derived carbon dots (S-agCDs). The antigen-antibody immunoreaction results in forming a core-satellite immunocomplex between anti-NoV antibody-conjugated S-agCDs and polydopamine-functionalized magnetic silver nanocubes [poly (dop)-MNPs-Ag NCs]. By deploying an immunomagnetic enrichment protocol and performing the SERS modality on a single-layer graphene substrate, norovirus-like particles (NoV-LPs) were detected across a wide range of 1 fg mL−1 – 10 ng mL−1 with an excellent limit of detection of 0.1 fg mL−1. The combined advantage of the dual-signaling properties of the biosensor was demonstrated using FL confocal imaging for “hotspots” tracking prior to SERS detection of clinical NoV in fecal specimen down to ⁓10 RNA copies mL−1. The proposed dual-modality biosensor's performance increases the prospect of a rapid and low-cost sensitive NoV detection and surveillance option for public health.

Original languageEnglish
Article number113540
JournalBiosensors and Bioelectronics
Publication statusPublished - 3 Aug 2021

Bibliographical note

Funding Information:
OJA gratefully thanks the Japan Society for the Promotion of Science ( JSPS ) for a Postdoctoral Fellowship for Research in Japan (Standard) (Grant No. 19F19348). The Japan Agency for Medical Research and Development partly supported this work (20hm0102080h0001).

Publisher Copyright:
© 2021 Elsevier B.V.


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