The impact of venepuncture training on the reduction of pre-analytical blood sample haemolysis rates: a systematic review

Nellie Makhumula-Nkhoma, Kathryn Weston, Robert McSherry, Greg Atkinson

Research output: Contribution to journalReview article

Abstract

Background: Venepuncture involves the introduction of a needle into a vein to collect a representative blood sample for laboratory testing. In the pre-analytical phase, haemolysis (the rupturing of erythrocytes and release of their contents into the extracellular compartment) has safety, quality and cost implications. Training in correct venepuncture practice has the potential to reduce in vitro haemolysis rates, but the evidence for this notion has yet to be synthesised.
Design: Systematic review.
Method: Published studies on the effectiveness of venepuncture training on haemolysis rates were searched in relevant databases. The McMaster critical appraisal tool was used to assess methodological quality. The GRADE tool was used to evaluate the body of evidence in relation to the research questions. Implementation Fidelity was also scrutinised in each study.
Results: Eight out of 437 retrieved studies met the inclusion criteria. None were randomised controlled trials (RCT). Between-study heterogeneity in design, intervention characteristics and the biochemical threshold for haemolysis precluded a meta-analysis. Post-training reductions in haemolysis rates of between 0.4-19.8% were reported in four of the studies, which developed their intervention according to a clear evidence-base and included mentoring in the intervention. Rises in haemolysis rates of between 1.3-1.9% were reported in two studies, while the intervention effect was inconsistent within two other studies.
Conclusion: There are no RCTS on the effectiveness of venepuncture training for reducing haemolysis rates, and findings from the existing uncontrolled studies are unclear. For a more robust evidence base, we recommend more RCTs with standardisation of haemolysis thresholds and training-related factors.
Relevance to clinical practice: While venepuncture training is an important factor influencing quality of blood sample in clinical practice, more robust evidence is needed to make specific recommendations about training content for reduction of haemolysis rates. Standardisation of haemolysis thresholds would also enable future meta-analyses.
Original languageEnglish
JournalJournal of Clinical Nursing
Early online date11 Jul 2019
Publication statusE-pub ahead of print - 11 Jul 2019

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Phlebotomy
Hemolysis
Meta-Analysis
Needles
Veins
Randomized Controlled Trials
Erythrocytes
Databases
Safety
Costs and Cost Analysis

Cite this

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title = "The impact of venepuncture training on the reduction of pre-analytical blood sample haemolysis rates: a systematic review",
abstract = "Background: Venepuncture involves the introduction of a needle into a vein to collect a representative blood sample for laboratory testing. In the pre-analytical phase, haemolysis (the rupturing of erythrocytes and release of their contents into the extracellular compartment) has safety, quality and cost implications. Training in correct venepuncture practice has the potential to reduce in vitro haemolysis rates, but the evidence for this notion has yet to be synthesised.Design: Systematic review.Method: Published studies on the effectiveness of venepuncture training on haemolysis rates were searched in relevant databases. The McMaster critical appraisal tool was used to assess methodological quality. The GRADE tool was used to evaluate the body of evidence in relation to the research questions. Implementation Fidelity was also scrutinised in each study. Results: Eight out of 437 retrieved studies met the inclusion criteria. None were randomised controlled trials (RCT). Between-study heterogeneity in design, intervention characteristics and the biochemical threshold for haemolysis precluded a meta-analysis. Post-training reductions in haemolysis rates of between 0.4-19.8{\%} were reported in four of the studies, which developed their intervention according to a clear evidence-base and included mentoring in the intervention. Rises in haemolysis rates of between 1.3-1.9{\%} were reported in two studies, while the intervention effect was inconsistent within two other studies. Conclusion: There are no RCTS on the effectiveness of venepuncture training for reducing haemolysis rates, and findings from the existing uncontrolled studies are unclear. For a more robust evidence base, we recommend more RCTs with standardisation of haemolysis thresholds and training-related factors.Relevance to clinical practice: While venepuncture training is an important factor influencing quality of blood sample in clinical practice, more robust evidence is needed to make specific recommendations about training content for reduction of haemolysis rates. Standardisation of haemolysis thresholds would also enable future meta-analyses.",
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year = "2019",
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language = "English",
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The impact of venepuncture training on the reduction of pre-analytical blood sample haemolysis rates: a systematic review. / Makhumula-Nkhoma, Nellie; Weston, Kathryn; McSherry, Robert; Atkinson, Greg.

In: Journal of Clinical Nursing, 11.07.2019.

Research output: Contribution to journalReview article

TY - JOUR

T1 - The impact of venepuncture training on the reduction of pre-analytical blood sample haemolysis rates: a systematic review

AU - Makhumula-Nkhoma, Nellie

AU - Weston, Kathryn

AU - McSherry, Robert

AU - Atkinson, Greg

PY - 2019/7/11

Y1 - 2019/7/11

N2 - Background: Venepuncture involves the introduction of a needle into a vein to collect a representative blood sample for laboratory testing. In the pre-analytical phase, haemolysis (the rupturing of erythrocytes and release of their contents into the extracellular compartment) has safety, quality and cost implications. Training in correct venepuncture practice has the potential to reduce in vitro haemolysis rates, but the evidence for this notion has yet to be synthesised.Design: Systematic review.Method: Published studies on the effectiveness of venepuncture training on haemolysis rates were searched in relevant databases. The McMaster critical appraisal tool was used to assess methodological quality. The GRADE tool was used to evaluate the body of evidence in relation to the research questions. Implementation Fidelity was also scrutinised in each study. Results: Eight out of 437 retrieved studies met the inclusion criteria. None were randomised controlled trials (RCT). Between-study heterogeneity in design, intervention characteristics and the biochemical threshold for haemolysis precluded a meta-analysis. Post-training reductions in haemolysis rates of between 0.4-19.8% were reported in four of the studies, which developed their intervention according to a clear evidence-base and included mentoring in the intervention. Rises in haemolysis rates of between 1.3-1.9% were reported in two studies, while the intervention effect was inconsistent within two other studies. Conclusion: There are no RCTS on the effectiveness of venepuncture training for reducing haemolysis rates, and findings from the existing uncontrolled studies are unclear. For a more robust evidence base, we recommend more RCTs with standardisation of haemolysis thresholds and training-related factors.Relevance to clinical practice: While venepuncture training is an important factor influencing quality of blood sample in clinical practice, more robust evidence is needed to make specific recommendations about training content for reduction of haemolysis rates. Standardisation of haemolysis thresholds would also enable future meta-analyses.

AB - Background: Venepuncture involves the introduction of a needle into a vein to collect a representative blood sample for laboratory testing. In the pre-analytical phase, haemolysis (the rupturing of erythrocytes and release of their contents into the extracellular compartment) has safety, quality and cost implications. Training in correct venepuncture practice has the potential to reduce in vitro haemolysis rates, but the evidence for this notion has yet to be synthesised.Design: Systematic review.Method: Published studies on the effectiveness of venepuncture training on haemolysis rates were searched in relevant databases. The McMaster critical appraisal tool was used to assess methodological quality. The GRADE tool was used to evaluate the body of evidence in relation to the research questions. Implementation Fidelity was also scrutinised in each study. Results: Eight out of 437 retrieved studies met the inclusion criteria. None were randomised controlled trials (RCT). Between-study heterogeneity in design, intervention characteristics and the biochemical threshold for haemolysis precluded a meta-analysis. Post-training reductions in haemolysis rates of between 0.4-19.8% were reported in four of the studies, which developed their intervention according to a clear evidence-base and included mentoring in the intervention. Rises in haemolysis rates of between 1.3-1.9% were reported in two studies, while the intervention effect was inconsistent within two other studies. Conclusion: There are no RCTS on the effectiveness of venepuncture training for reducing haemolysis rates, and findings from the existing uncontrolled studies are unclear. For a more robust evidence base, we recommend more RCTs with standardisation of haemolysis thresholds and training-related factors.Relevance to clinical practice: While venepuncture training is an important factor influencing quality of blood sample in clinical practice, more robust evidence is needed to make specific recommendations about training content for reduction of haemolysis rates. Standardisation of haemolysis thresholds would also enable future meta-analyses.

M3 - Review article

JO - Journal of Clinical Nursing

JF - Journal of Clinical Nursing

SN - 0962-1067

ER -