TY - JOUR
T1 - The role of APC-mediated actin assembly in microtubule capture and focal adhesion turnover
AU - Juanes, M. Angeles
AU - Isnardon, Daniel
AU - Badache, Ali
AU - Brasselet, Sophie
AU - Mavrakis, Manos
AU - Goode, Bruce L.
PY - 2019/10/7
Y1 - 2019/10/7
N2 - Focal adhesion (FA) turnover depends on microtubules and actin. Microtubule ends are captured at FAs, where theyinduce rapid FAdisassembly. However,actin’srolesare less clear. Here, we usepolarization-resolved microscopy,FRAP, livecell imaging, anda mutant of Adenomatous polyposis coli(APC-m4) defective in actin nucleationtoinvestigatethe roleof actinassemblyin FA turnover. Weshow that APC-mediated actin assembly is criticalfor maintaining normalF-actinlevels,organization,and dynamics at FAs, along with organization of FA components. In wild type cells,microtubulesarecaptured repeatedlyat FAsasthey mature, butoncea FA reaches peak maturity,the next microtubule capture event leads todelivery of an autophagosome, triggering FA disassembly. InAPC-m4 cells,microtubule capture frequency and duration are altered, and there arelong delays between autophagosome delivery and FA disassembly. Thus, APC-mediated actin assemblyis required for normalfeedback between microtubules and FAs, and maintainingFAsin astate‘primed’ for microtubule-induced turnover.
AB - Focal adhesion (FA) turnover depends on microtubules and actin. Microtubule ends are captured at FAs, where theyinduce rapid FAdisassembly. However,actin’srolesare less clear. Here, we usepolarization-resolved microscopy,FRAP, livecell imaging, anda mutant of Adenomatous polyposis coli(APC-m4) defective in actin nucleationtoinvestigatethe roleof actinassemblyin FA turnover. Weshow that APC-mediated actin assembly is criticalfor maintaining normalF-actinlevels,organization,and dynamics at FAs, along with organization of FA components. In wild type cells,microtubulesarecaptured repeatedlyat FAsasthey mature, butoncea FA reaches peak maturity,the next microtubule capture event leads todelivery of an autophagosome, triggering FA disassembly. InAPC-m4 cells,microtubule capture frequency and duration are altered, and there arelong delays between autophagosome delivery and FA disassembly. Thus, APC-mediated actin assemblyis required for normalfeedback between microtubules and FAs, and maintainingFAsin astate‘primed’ for microtubule-induced turnover.
U2 - 10.1083/jcb.201904165
DO - 10.1083/jcb.201904165
M3 - Article
SN - 0021-9525
VL - 218
SP - 3415
EP - 3435
JO - Journal of Cell Biology
JF - Journal of Cell Biology
IS - 10
ER -
