Urine markers and formula for diagnosing overactive bladder disorder

The present invention relates to a method of diagnosing over active bladder disorder (OAB), the method comprising: measuring the concentrations of adenosine triphosphate (ATP), acetylcholine (ACh), nitrite, monocyte chemoattractant protein 1 (MCP-1) and interleukin 5 (IL-5) in a sample obtained from a subject; normalising the concentrations to the concentration of creatinine (Cr) in the sample; range standardising the normalised concentrations and subject's age to the following values: Age to 120 years old; ATP/Cr to 0.000001 mole/mg×dl−1; ACh/Cr to 0.1 mole/mg×dl−1; Nitrite to 200 nM/mg×dl−1; MCP-1/Cr to 100 pg×ml−1/mg×dl−1; IL-5/Cr 100 pg×ml-mg×dl−1; applying the normalised and range standardised concentrations to the following formula: Logit (p)=−1.738±1.404±4.985±2.914×subject's age+3315.959±5435.254×[ATP]/[Cr]+(−25204.194±20268.337)×[ACh]/[Cr]+26.799±32.967×[nitrite]/[Cr]+6.755±25.132 [MCP-1]/[Cr]+(−61.838±148.740) [IL-5]/[Cr] and calculating Logit; wherein a Logit value above a predetermined threshold indicates that the subject has OAB. Methods for monitoring the progression of OAB using the method, kits for use in the method and computer systems and programs configured to execute the method are also provided.