We investigated the effects of supplementing 20 healthy adults with WKBE for one week in a randomised, placebo-controlled crossover trial on the composition of the gut microbiota, gastrointestinal inflammation, gastrointestinal symptoms, and stool consistency/frequency. We also conducted in vitro experiments and used a novel gut model system to explore potential inhibition of alpha-amylase, and gained qualitative insight into participant experiences of using WKBE via focus groups. WKBE supplementation for seven days significantly decreased the relative abundance of Bacteroidetes and increased the relative abundance of Firmicutes, however there were no significant differences in post-intervention gut microbiota measurements between the WKBE and Control treatment periods. Further, there were no significant effects on gastrointestinal inflammation (faecal calprotectin) or symptoms related to constipation (e.g., abdominal pain, discomfort or bloating, or constipation), or stool consistency or frequency. Our in vitro and gut model system analyses showed no effects of WKBE on alpha-amylase activity. Altogether, our findings suggest that WKBE may modulate the gut microbiota in healthy adults, however the underlying mechanism is unlikely due to active site inhibition of alpha-amylase.
|Publication status||Published - 1 Jun 2023|